Objective To investigate Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China. Methods A total of 33 rodents were captured by night trapping and cage method, and their spleen were aseptically collected to detect Bartonella by PCR. The positive samples obtained were further identified through gene sequencing. Results The results showed that 6 positive samples of Bartonella in all rodents,infection prevalence was 18.18%, 5 of which were isolated from Apodemus agrarius, one from Rattus norvegicus. Results showed that at least 4 subtypes of Bartonella were found at Mudanjiang airport, including B. taylorii, B. phoceensis, B. grahamii that are pathogenic to humans, as well as an undetermined species. The results suggested the genotype diversity of Bartonella at Mudanjiang airport. Conclusion The prevalence of Bartonella infection in rodents at Mudanjiang airport poses a risk of transmission to human, and appropriate control measures should be taken into consideration.

Objective To investigate ticks that harbor emerging tick-borne pathogens and co-infection in lava area of Xunke county of Heilongjiang. Methods A total of 257 live ticks were collected in April to June, 2015. All samples were amplified for specific fragments of 6 emerging tick-borne pathogens by PCR, and further identified through gene sequencing. Results The results showed that 86 positive samples of spotted fever group Rickettsia, infection prevalence was 33.46%; Five positive samples of Bartonella, infection prevalence was 1.94%; 11 positive samples of Anaplasma phagocytophilum, infection prevalence was 4.28%; DNA of Borrelia burgdorferi, Babesia, Ehrlichiosis detection results were all negative. It was also found that 8 co-infection samples existed in Dermacentor silvarum, Ixodes persuleatu, and Haemaphysalis concinna, and the infection prevalence was 3.11%. It was confirmed that co-infection of spotted fever group Rickettsia and Bartonella existed in D. silvarum and coinfection of spotted fever group Rickettsia and An. phagocytophilum existed in I. persuleatus and H. concinna. Conclusion To strengthen the prevention and control of vector ticks in lava area of Xunke county of Heilongjiang is warranted.

Objective To investigate rodent-borne pathogens in volcano lava areas of Xunke county in Heilongjiang, China. Methods A total of 107 rodents were collected during April to September in 2014, and necropsy was conducted to acquire lung, kidney, liver, and kidney and bladder samples. All samples were amplified for the specific fragments of 10 rodent - borne pathogens by PCR, and further identified by gene sequencing. Results Five positive samples of SEO Hantavirus RNA were detected from 107 rodent's lung tissues, infection prevalence was 4.67%; 6 positive samples of leptospirosis DNA were detected from 107 rodent's kidney tissues, infection prevalence was 5.61%; 7 positive samples of Bartonella and 4 positive samples of Anaplasma phagocytophilum were detected from 107 rodent's spleen tissues, the prevalence of infection were 6.54% and 3.74% respectively; Detection of Yersinia pestis, Babesia, Spotted fever group rickettsia, Rickettsia tsutsugamushi, Rickettsia mooseri from rodent's liver and spleen tissues and Borrelia burgdorferi from rodent's Bladder was all negative. Multiple infection was found in some rodents, the infection prevalence was 2.80%. Conclusion Hantavirus, Leptospirosis, Bartonella, Anaplasma phagocytophilum exist in rodent populations that dwell in the volcano lava areas of Xunke county, Heilongjiang, China.

【Abstract】 Objective To investigate control effect of flies in temporary shelters of post-earthquake disaster areas and to provide the science evidence for its control. Methods Manage environmental sanitation and spray periodically insecticide to control flies in temporary shelters. Investigate fly population by cage-trap method, monitor flies density by eye balling method in washrooms, trash piles(cans), inside and outside of tents, and survey the management of flies breeding sites and prevention and control measure of civilians by field observation. Results Houseflies was the dominant species （96.85%） in the temporary shelters. The average fly density was less than one fly/m2 around temporary shelters after the earthquake for 4-8 weeks. At the fifth week post-earthquake, all the shelters were equipped with washroom. About 76.92% trash containers were equipped, and 69.23% of domestic garbage were cleaned up and transported away without delay. 94.01% of food-leavings was covered, and 93.00% of tableware was deposited in the cupboard. Conclusion It was effective to take the integrated pest management measures to control flies in the temporary shelters. The control of breeding sites should be strengthened in the future.